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Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, <t>Smad2/3,</t> Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.
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Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, <t>Smad2/3,</t> Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.
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Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, <t>Smad2/3,</t> Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.
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Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, <t>Smad2/3,</t> Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.
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Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, <t>Smad2/3,</t> Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.
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Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, <t>Smad2/3,</t> Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.
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Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, <t>Smad2/3,</t> Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.
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Image Search Results


Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, Smad2/3, Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.

Journal: ACS Pharmacology & Translational Science

Article Title: Nesfatin‑1 Deficiency Exacerbates Calcium Oxalate Monohydrate–Induced Cellular Injury in Renal Epithelial Cells

doi: 10.1021/acsptsci.6c00200

Figure Lengend Snippet: Immunofluorescence analysis of EMT and fibrosis markers in NRK-52E cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, Smad2/3, Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.

Article Snippet: Primary antibodies against p–NF–κB (sc-101,749) Caspase-3 (sc-7272), α-SMA (sc-53142), E-cadherin (sc-8426), Vimentin (sc-6260), Smad2/3 (sc-133098), Smad4 (sc-7966), and GAPDH (sc-137179), along with corresponding secondary antibodies (sc-2357 and sc-516102), were purchased from Santa Cruz Biotechnology (CA, USA).

Techniques: Immunofluorescence, Analysis, Expressing, Control

Immunofluorescence analysis of EMT and fibrosis markers in PRTE cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, Smad2/3, Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; $ p < 0.05 vs respective ASO + COM groups; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.

Journal: ACS Pharmacology & Translational Science

Article Title: Nesfatin‑1 Deficiency Exacerbates Calcium Oxalate Monohydrate–Induced Cellular Injury in Renal Epithelial Cells

doi: 10.1021/acsptsci.6c00200

Figure Lengend Snippet: Immunofluorescence analysis of EMT and fibrosis markers in PRTE cells. (A–F) Representative images and quantification of TGF-β1, α-SMA, Smad2/3, Smad4, vimentin, and E-cadherin expression across treatment groups. (G) Quantitative mean immunofluorescence. Data are expressed as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs respective control groups; # p < 0.05 vs respective COM group; $ p < 0.05 vs respective ASO + COM groups; ^ p < 0.05 vs respective Scr-ASO + COM groups; ns = nonsignificant.

Article Snippet: Primary antibodies against p–NF–κB (sc-101,749) Caspase-3 (sc-7272), α-SMA (sc-53142), E-cadherin (sc-8426), Vimentin (sc-6260), Smad2/3 (sc-133098), Smad4 (sc-7966), and GAPDH (sc-137179), along with corresponding secondary antibodies (sc-2357 and sc-516102), were purchased from Santa Cruz Biotechnology (CA, USA).

Techniques: Immunofluorescence, Analysis, Expressing, Control